Protocols

Schistosomes

Developmental Stages (Schistosoma mansoni, Schistosoma haematobium)

• Cercarial transformation
• Isolation of eggs
• In vitro laid eggs (IVLE)
• Cercarial transformation - from a single snail
• Sporocysts
• Adult worms, maintenance in vitro
• PI/FDA staining

Genomic protocols

• Isolation of genomic DNA from a single worm
• Transgenesis using retrovirus Murine Leukemia Virus (MLV)
• Transgenesis – lentivirus (LV)
• Transgenesis using the piggyBac transposon
• Conventional RNAi
• Vector based RNAi
• Whole worm In Situ Hybridization (WISH)
• Confocal microscopy
• Protein protocols
• Protein isolation
• Luciferase activity assays
•  Cathepsin activity

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Opisthorchis viverrini

• Protein chemistry
• In situ labeling

Fasciola hepatica

• In vitro excystment of metacercariae
• Newly excysted juvenile collection, culture and manipulation

Snail intermediate hosts of schistosomes and other trematodes

• Bge Cells in culture